Journal: iScience

Article Title: Oleic acid triggers metabolic rewiring of T cells poising them for T helper 9 differentiation

doi: 10.1016/j.isci.2024.109496

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Article Snippet: Anti-human IL-22; Vio® B515; Isotype Human; Clone REA466 , Miltenyi Biotec , Cat#130-108-096; RRID: AB_2652431.

Techniques: Activation Assay, Control, Isolation, Recombinant, Modification, Staining, cDNA Synthesis, Gene Expression, Software

Journal: Immunity

Article Title: Low-Avidity CD4 + T Cell Responses to SARS-CoV-2 in Unexposed Individuals and Humans with Severe COVID-19

doi: 10.1016/j.immuni.2020.11.016

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Article Snippet: IL-21-PE (REA1039) , Miltenyi Biotec , Cat#130-117-421; RRID: AB_2727941.

Techniques: Functional Assay, Recombinant, Staining, Sequencing, Gene Expression, Software

Journal: eLife

Article Title: Transcriptional correlates of malaria in RTS,S/AS01-vaccinated African children: a matched case–control study

doi: 10.7554/eLife.70393

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Article Snippet: Antibody , anti-IL-21, clone 3A3-N2 (mouse monoclonal) , Miltenyi Biotec , Cat# 130-120-702 , Fluorochrome: APC.

Techniques: Staining, Concentration Assay, Recombinant, Produced, Sequencing, Isolation, Gel Extraction, Software, Adhesive, Reverse Transcription, Magnetic Beads

Journal: Cell reports

Article Title: Single-cell RNA sequencing identifies a population of human liver-type ILC1s

doi: 10.1016/j.celrep.2022.111937

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: IL22 APC (REA466) , Miltenyi , RRID: AB_2652429; Cat#130-106-959.

Techniques: Blocking Assay, Recombinant, Clinical Proteomics, Nucleic Acid Electrophoresis, Software, Inverted Microscopy

Journal: Journal of Immunology Research

Article Title: Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice

doi: 10.1155/2021/6630715

Figure Lengend Snippet: Metoclopramide increases the absolute number of T FH populations in MRL/lpr mice. Nine-week-old MRL/lpr mice were treated with metoclopramide (meto), bromocriptine (bromo), or PBS or were left without intervention (left column marked by age in weeks) for 6 weeks. (a) Flow chart of treatment strategy. At the end of the treatment, cells were labeled with anti-CD4, anti-CXCR5, anti-PD1, anti-OX40, anti-BCL6, or anti-IL21 antibodies. The graphs show the absolute number of (b) splenocytes, (c) CD4 + T cells, (d) T FH cells, (e) OX40 + T FH cells, and (f) IL21 + T FH cells. (g) Expression of BCL6 in T FH cells. (h) IL21 concentration in serum. For the determination of T FH IL21 + , the cells were stimulated with stimulation cocktail (PMA ionomycin) for 5 h and then stained. MFI: mean fluorescent intensity. Eight mice per condition were used. Pooled data are presented as the mean ± SD; ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05 using ANOVA.

Article Snippet: The antibodies used for cell culture were as follows: anti-CD3 (clone 145-2C11) and anti-CD28 (clone 37.51) from Invitrogen, USA; anti-IFN- γ (clone XMG12) and anti-IL4 (clone 11B11) from BioLegend, USA; anti-TGF β from Peprotech, USA; cytokines IL6 and IL21 from Miltenyi Biotec, Germany.

Techniques: Labeling, Expressing, Concentration Assay, Staining

Journal: Journal of Immunology Research

Article Title: Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice

doi: 10.1155/2021/6630715

Figure Lengend Snippet: Activation and signaling of differentiated T FH cells in vitro in the presence of PRL. T naïve cells from 9-week-old C57BL/6 and MRL/lpr mice were purified by MACS and differentiated to T FH with or without PRL. (a) Expression of OX40 (MFI) and representative histograms of OX40 expression in T FH cells from MRL/lpr mice. (b) Percentage and representative Zebra plots of T FH OX40 + cells. (c) Expression of IL21 (MFI) and representative histogram of IL21 expression in T FH cells from MRL/lpr mice. (d) Percentage and Zebra plots of T FH IL21 + cells. (e, f) T naïve and T FH cells were preincubated for 30 min with the inhibitor of STAT3 (Stattic). For STAT3 inhibition, T naïve cells were differentiated to T FH , left to rest for 8 h, and then incubated for 30 min with PRL to subsequently determine the MFI of pSTAT3 (histogram). For the plot, pSTAT3 is reported as fold change taken as baseline levels found in T naïve or T FH cells only treated with medium. (f) Percentage and Zebra plots of pSTAT3. Six different experiments were performed; each experiment was done in duplicate. Pooled data are presented as the mean ± SD; ∗∗∗ p < 0.0001 and ∗ p < 0.05 using ANOVA.

Article Snippet: The antibodies used for cell culture were as follows: anti-CD3 (clone 145-2C11) and anti-CD28 (clone 37.51) from Invitrogen, USA; anti-IFN- γ (clone XMG12) and anti-IL4 (clone 11B11) from BioLegend, USA; anti-TGF β from Peprotech, USA; cytokines IL6 and IL21 from Miltenyi Biotec, Germany.

Techniques: Activation Assay, In Vitro, Purification, Expressing, Inhibition, Incubation

Journal: Journal of Immunology Research

Article Title: Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice

doi: 10.1155/2021/6630715

Figure Lengend Snippet: Working model of the mechanism of action of PRL in T FH cells from mice that developed SLE. T FH cells from lupus-prone mice display increased levels of PRL receptor expression restrictive to the long isoform, together with higher levels of serum PRL. This combination results in the heightened activation of STAT3, as well as the increase in OX40 expression and IL21 secretion, which increase the activation of T FH cells favoring the formation of germinal centers, generation of autoreactive plasma cells and increased levels of autoantibodies, with enhanced tissue damage due to the immune complex deposition that characterizes SLE.

Article Snippet: The antibodies used for cell culture were as follows: anti-CD3 (clone 145-2C11) and anti-CD28 (clone 37.51) from Invitrogen, USA; anti-IFN- γ (clone XMG12) and anti-IL4 (clone 11B11) from BioLegend, USA; anti-TGF β from Peprotech, USA; cytokines IL6 and IL21 from Miltenyi Biotec, Germany.

Techniques: Expressing, Activation Assay, Clinical Proteomics